Controlling proteins the intein way
نویسندگان
چکیده
منابع مشابه
Facile chemical functionalization of proteins through intein-linked yeast display.
Intein-mediated expressed protein ligation (EPL) permits the site-specific chemical customization of proteins. While traditional techniques have used purified, soluble proteins, we have extended these methods to release and modify intein fusion proteins expressed on the yeast surface, thereby eliminating the need for soluble protein expression and purification. To this end, we sought to simulta...
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InBase, the Intein Database (http://www.neb.com/neb/inteins.html ), is a comprehensive on-line resource that includes the Intein Registry. Inteins are protein splicing elements that mediate a self-catalytic protein splicing reaction. InBase presents general information as well as detailed data for each intein, including tabu-lated comparisons and a comprehensive bibliography.
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2007
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth0207-112a